336 MANUAL OF BACTERIOLOGY. 



ease of horses and mules occurring in India and the Philippine islands) 

 and of the tsetse-fly disease of South Africa; while others are found 

 in rats, birds, amphibia and fishes. In the horse the infection is trans- 

 mitted by the bites of flies. Novy and McNeil have succeeded in culti- 

 vating the trypanosoma of rats on rabbit blood-agar. 1 



Several cases were reported during 1902 where trypanosomes were 

 found in the b ood of individuals from tropical Africa, showing that 

 this group of parasites may occur in man. 2 The symptomatology of 

 these infections requires further study. Still more recently it has been 

 claimed by Castellani that a trypanosoma is the cause of " sleeping sick- 

 ness," a disease of the natives of Africa. He states that the parasites 

 may be demonstrated in the cerebro-spinal fluid obtained by lumbar 

 puncture and, with greater difficulty, in the blood, during life. Many 

 cases also show at autopsy streptococcus infection, which is believed to 

 be a secondary invasion. 3 



1 Novy and McNeil, in Contributions to Medical Research dedicated 

 to Victor C. Vaughan, 1903. 



2 British Medical Journal, May 30, 1903. 



3 Britisli Medical Journal and Lancet, June 20, 1903. 



