362 MISSOURI AGRICULTURAL EXP. STA., RESEARCH BULLETIN NO. IO 



washed out with a stream of carbon bisulphide. They were all 

 washed out however, by a one per cent alcoholic petroleum ether 

 solution. 



Bleached Alfalfa Hay. This hay was quite free from green 

 stalks and as it had been found palatable to the cows it was of special 

 interest as an experimental roughage for non-pigmented feeding 

 studies. A selected sample of the hay was ground up fine, and 

 extracted with ten per cent alcoholic petroleum ether. The light 

 grqen colored extract showed no indication of either carotin or 

 xanthophyll when analyzed by means of the chromotogramm, and no 

 yellow pigment was extracted from its alcoholic solution by petro- 

 leum ether or by carbon bisulphide. 



Yellow Corn. The unsaponifiable yellow pigment of yellow corn 

 is in all probability in the oil. It was found to be composed of two 

 constituents, the largest part of which is a xanthophyll-like pigment, 

 showing absorption bands in alcoholic and carbon bisulphide solu- 

 tions lying close to the normal xanthophyll bands. In CS 2 the bands 

 measured: I, 229-251; II, 274-291; III, not measured. It was not 

 adsorbed from either petroleum ether or carbon bisulphide by CaCO 3 

 but passed through as a yellow or orange zone. Its carbon bisulphide 

 solutions were orange colored. Petroleum ether readily extracted 

 the pigment from its concentrated eighty per cent alcoholic solu- 

 tion, but it could be completely re-extracted from its petroleum ether 

 solution by fresh eighty per cent alcohol. In this respect it differed 

 from any xanthophyll-like pigment yet investigated. The pigment 

 was more soluble in carbon bisulphide than in eighty per cent alcohol 

 and in this respect favored carotin. On warming its alcoholic solu- 

 tion containing a little concentrated hydrochloric acid, the color of 

 the solution changed to a light greenish blue, with the fading of 

 the absorption bands. The minor constituent of the corn pigment 

 had the spectroscopic, solubility and adsorption properties of carotin. 



The Carrot. It was planned to conduct some feeding experiments 

 with carrots, and a special study of its relative proportion of carotin and 

 xanthophyll was accordingly considered advisable. A large well- 

 colored carrot was scraped, chopped fine, and boiled in water for 

 one hour. The softened tissue was poured onto cheesecloth and 

 the water squeezed out of the pulp. The pulp was pressed through 

 a wire gauze, dried and powdered. The meal was extracted with 

 carbon bisulphide and a portion of the blood red solution analyzed 

 by means of a chromotogramm. By far the greatest part of the pig- 

 ment passed through unadsorbed as a rose colored zone, leaving a 

 small amount of adsorbed pigment in the column which was not 

 differentiated into zones but which was readily washed out with alco- 



