CAROTIN, THE PRINCIPAL YELLOW PIGMENT OF MILK FAT 37! 



TABLE No. 13. EFFECT OF A LONG-CONTINUED FEEDING OF A NON-PIGMENTED 



RATION UPON THE COLOR OF MILK FAT. AYRSHIRE 



Cow No. 301. 



It will be of interest to state in connection with this experiment 

 that it furnished the three "light colored" fats for the studies of the 

 proportion of carotin and xanthophyll which were reported above. 



Experiment 4. 



The color of the butter 'fat of Cow No. 301 was now so low that 

 the conditions were considered ideal for one or two additional im- 

 portant investigations, first a confirmation of the apparently negative 

 effect of feeding yellow corn which was obtained in Experiments I 

 and 2, and second a study of carrot feeding. In the latter study the 

 results would be especially interesting in view of the fact that the 

 pigment fed would be almost pure carotin. The hay used in this 

 experiment was a very light colored timothy hay which was not quite 

 so free from carotin and xanthophylls as the bleached alfalfa of Expe- 

 riment 3, but which apparently had no effect on the color of the fat 



