396 MISSOURI AGRICULTURAL EXP. STA V RESEARCH BULLETIN NO. II 



cent alcohol than in petroleum ether (b. p. 30-50). This constituent, 

 when combined with the pigment adsorbed by the CaCO 3 in the 

 chromotogramm, did not show sufficiently sharp absorption bands 

 for accurate measurements. 



The main petroleum ether soluble pigment was transferred to 

 carbon bisulphide in which it showed two strong bands and a third 

 faint one, the measurements of which are given in Table 2. The 

 residue from this solution gave a greenish-blue color with concen- 

 trated sulphuric acid. 



, Experiment II. 



An unusually high colored fat taken from the back of a Jersey 

 cow was used for this experiment. The pure rendered fat tested 

 in the one-inch tintometer cell 80 yellow, 2.7 red. Thirty grams 

 of this fat was saponified with 300 c. c. of 5 per cent alcoholic pot- 

 ash by dissolving the fat in the hot alkaline solution, letting stand 

 for 24 hours in the cold with frequent shaking and finally boiling on 

 the steam bath for one half hour. By using this procedure not a 

 trace of foreign pigment was developed. One extraction with ether 

 rendered the diluted soap colorless. The golden yellow ether ex- 

 tract, after purification, was concentrated into 50 c. c. of absolute 

 alcohol. On careful analysis of this solution it was found possible 

 to separate its pigment into a major pigment which was readily ex- 

 tracted by petroleum ether and a minor pigment which could not be 

 extracted by petroleum ether. The main petroleum ether soluble 

 pigment was readily soluble in carbon bisulphide with a blood red 

 color, and in this solvent showed two strong absorption bands and 

 a third faint one, the measurements of which are given in Table 2. 

 Analyzed chromotographically, the carbon bisulphide solution passed 

 through as an unadsorbed beautiful rose colored zone. There was 

 no differentiation. The residue from the solution gave a deep blue 

 color with concentrated sulphuric acid. 



The alcohol soluble pigment, which probably comprised several 

 per cent of the total, was transferred to ether by diluting the alco- 

 holic solution with much water in a separatory funnel. Petroleum 

 ether was added, precipitating some water, and the ethereal solu- 

 tion washed with water until clear. The solution was now evap- 

 orated and the yellow residue dissolved in carbon bisulphide, giving 

 a yellow-orange solution which showed two fine absorption bands, and 

 a third fainter one, the measurements of which are given in Table 

 2. The bands seem to be shifted more toward the blue than the 

 usual xanthophyll bands. 



