448 MISSOURI AGRICULTURAL EXP. STA. RESEARCH BULLETIN NO. 12 



showed two beautiful absorption bands, and possibly a third. The 

 CS 2 was carefully evaporated. A part of the residue which remained 

 was difficultly soluble in absolute alcohol, but readily dissolved when 

 a little petroleum ether was added. When differentiated between 

 petroleum ether and 80 per cent alcohol the combined pigment was 

 readily divided into two apparently equal proportions with perhaps 

 slightly more color in the petroleum ether layer. 



The pigment of the petroleum ether layer gave a red-orange 

 carbon bisulphide solution showing two strong absorption bands and 

 a third faint one, the measurements of which are given in Table No. i 

 below. j 



TABLE No. 1. ABSORPTION BANDS OF CAROTIN AND XANTHOPHYLLS FROM 

 HUMAN MILK FAT. 



The pigment of the alcoholic layer gave a yellow-orange, carbon 

 bisulphide solution showing two good absorption bands and end absorp- 

 tion, the measurements of which are given in Table i. 



Experiment No. 2 



As stated above, this sample of human milk was taken by one 

 of us and represented the milk of the first few days of lactation 

 including the colostrum milk. The milk itself was characterized 

 by a high yellow color and the fat which rose to the top of the 

 sample had a very deep yellow color. About 350 c.cm. of milk were 

 obtained. The fat percentage being between 5 and 6, nearly 20 

 grams of fat were yielded for the study of the pigments. 



The fat was obtained from this sample of milk in a manner 

 very similar to that used in the preceding experiment. The proteins 

 and fat were precipitated together by adding a little salt and also con- 



