6 



is f;ivon off. A few iniiiutes later the second Dolar body is 

 forrnt^d. They remain near the egg for some tiihO; frequently 

 until after the second or third segirientatior; . The oolar 

 bodies are not held by a merribrane, as the egp is devoid of 

 such a structure; neither are there any pi'Otorlasmic con- 

 nections visible vath a inagnif ication of 212 diarreters. Yet 

 for a tine they seem to be held near the egg by so^e r.eans 

 of attr^tction. The first oolar body -ay segment once or 

 twice. Usually about the time of the second cleavage the 

 polar bodies eiiher disintegrate or pass out into the water 

 and are lost. 



JSRTILIZATIOl^i. 



Very little concerning fertilization could be -.Tiade out 

 on account of the character of the eg?. The ova and spcrn^aia- 

 zoa are discharged irto the water and there fertilization 

 takes Place. It is i-::ossible to folio., the nuclear changes 

 which take olace during iiaturation; or the union of the rr;ale 

 and fen,ale proruclei in the living ege because oi the Jensity 

 of the cytorlas.T, and material could not be secured in suf- 



' A 



ficient abundance in the various phases for the preservation 



