98 PHYSIOLOGICAL CHEMISTRY. 



stratify with the formation of a white zone of precipitated albumin 

 at the point of juncture. This test is a modification of Heller's 

 ring test and is rather more satisfactory. The albumoscope may 

 also be used in making this test. (See page 97.) 



6. Spiegler's Ring Test. Place 5 c.c. of Spiegler's reagent 1 

 in a test-tube, incline the tube, and by means of a pipette allow 

 5 c.c. of albumin solution, acidified with acetic acid, to flow slowly 

 down the side. A white zone will form at the point of contact. 

 This is an exceedingly delicate test, in fact, too delicate for ordi- 

 nary clinical purposes, since it serves to detect albumin when present 

 in the merest trace (i 1250,000). This test is further discussed on 

 page 316. 



7. Jolles' Reaction. Shake 5 c.c. of albumin solution with i 

 c.c. of 30 per cent acetic acid and 4 -c.c. of Jolles' reagent 2 in a 

 test-tube. A white precipitate of albumin should form. Care 

 should be taken to use the correct amount of acetic acid. For 

 further discussion of the test see page 316. 



8. Tanret's Test. To 5 c.c. of albumin solution in a test-tube 

 add Tanret's reagent, 3 drop by drop, until a turbidity or precipitate 

 forms. This is an exceedingly delicate test. Sometimes the al- 

 bumin solution is stratified upon the reagent as in Heller's or Rob- 

 erts' ring tests. In urine examination it is claimed by Repiton that 

 the presence of urates lowers the delicacy of the test. Tanret has 

 however very recently made a statement to the effect that the re- 

 moval of urates is not necessary inasmuch as the urate precipitate 

 will disappear on warming and the albumin precipitate will not. He 

 says, however, that mucin interferes with the delicacy of his test 

 and should be removed by acidification with acetic acid and filtration 

 before testing for albumin. 



1 Spiegler's reagent has the following composition : 



Tartaric acid 20 grams. 



Mercuric chloride 40 



Glycerol TOO 



Distilled water 1000 " 



3 Jolles' reagent has the following composition: 



Succinic acid 40 grams. 



Mercuric chloride 20 " 



Sodium chloride 20 



Distilled water 1000 " 



8 Tanret's reagent is prepared as follows: Dissolve 1.35 gram of mercuric 

 chloride in 25 c.c. of water, add to this solution 3.32 grams of potassium iodide 

 dissolved in 25 c.c. of water, then make the total solution up to 60 c.c. with 

 water and add 20 c.c. of glacial acetic acid to the combined solutions. 



