

BILE. 



l6l 



benzoyl chloride can be detected. Cool, acidify with hydrochloric 

 acid, add an equal volume of petroleum ether and shake thoroughly 

 to remove the benzoic acid. (Evaporate this solution and note 



FIG. 44. 



GLYCOCOLL. 



the crystals of benzoic acid. Compare them with those shown 

 in Fig. 94, page 289.) Decant the ethereal solution into a porcelain 

 dish and extract again with ether. The hippuric acid remains in 

 the aqueous solution. Filter it off and wash it with a small amount 

 of cold water while still on the filter. Remove it to a small, shallow 

 vessel, dissolve it in a small amount of hot water and set it aside for 

 crystallization. Examine the crystals microscopically and com- 



ire them with those in Fig. 92, page 282. 



The chemistry of the synthesis is represented thus : 



COC1 OC-NH-CH 2 -COOH. 



/\ 



CH-NH 



OOH \/ \/ 



Glycocoll. Bcnzoyl chloride. Hipptiric acid. 



HC1. 



12 



