2O4 



PHYSIOLOGICAL CHEMISTRY. 



of some of the loosely combined oxygen from the oxy haemoglobin. 

 Examine this haemoglobin spectroscopically. Note that in place of 

 the two absorption bands of oxyhsemoglobin we now have a single 



ANGULAR-VISION SPECTROSCOPE ARRANGED FOR ABSORPTION ANALYSIS. 



broad band lying almost entirely between D and E. This is the 

 typical spectrum of haemoglobin. If the solution showing this 

 spectrum be shaken in the air for a few moments it will again as- 

 sume the bright red color of oxyhamioglobin and show the char- 

 acteristic spectrum of that pigment. 



FIG. 63. 



DIAGRAM OF ANGULAR-VISION SPECTROSCOPE. (Long.} 



The white light F enters the collimator tube through a narrow slit and passes 

 the prism P, which has the power of refracting and dispersing the light. The ra) 

 then pass to the double convex lens of the ocular tube and are deflected to the eye- 

 piece E. The dotted lines show the magnified virtual image which is formed. The 

 third tube contains a scale whose image is reflected into the ocular and shown with 

 the spectrum. Between the light' F and the collimator slit is placed a cell to hold 

 the solution undergoing examination. 



