234 



PHYSIOLOGICAL CHEMISTRY. 



for calcium and phosphates. To the precipitate remaining un- 

 dissolved on the paper add a little dilute hydrochloric acid and test 

 this last filtrate for phosphates and iron. 



Reference to the following scheme may facilitate the analysis. 



BONE ASH. 



Add dilute nitric acid, stir thoroughly and after the major portion of the ash 

 has been brought into solution add a little distilled water and filter. 



Residue I. 



(discard) 



Filtrate I. 



Add ammonium hy- 

 droxide to alkaline re- 

 action and filter. 



V. ADIPOSE TISSUE/ 

 For discussion and experiments see the chapter on Fats, page 131. 



