URINE. 2/5 



plish-violet color due to the production of the potassium salt is ob- 

 tained. The color disappears upon warming; with certain related 

 bodies (purine bases) the color persists under these conditions. 



5. Moreigne's Reaction. To equal volumes of Moreigne's re- 

 agent 1 and the solution to be tested add a few drops of concen- 

 trated potassium hydroxide. A blue color indicates the presence of 

 uric acid. 



6. Schiff 's Reaction. Dissolve a small amount of pure uric acid 

 in sodium carbonate solution and transfer a drop of the resulting 

 mixture to a strip of filter paper saturated with argentic nitrate 

 solution. A yellowish-brown or black coloration due to the forma- 

 tion of reduced silver is produced. 



7. Influence upon Fehling's Solution. Dilute i c.c. of Feh- 

 ling's solution with 4 c.c. of water and heat to boiling. 'Now add 

 sloivly, a few drops at a time, 1-2 c.c. of a concentrated solution of 

 uric acid in potassium hydroxide, heating after each addition. 

 From this experiment what do you conclude regarding the possi- 

 bility of arriving at an erroneous decision when testing for sugar 

 in the urine by means of Fehling's test? 



8. Reduction of Nylander's Reagent. To 5 c.c. of a solution 

 of uric acid in potassium hydroxide add about one-half a cubic 

 centimeter of Nylander's reagent and heat to boiling for a few mo- 

 ments. Do you obtain the typical black end-reaction signifying the 

 reduction of the bismuth ? 



-CO 



C = 



CREATININE, C = NH 



N - CH 3 CH 2 . 



Creatinine is the anhydride of creatine and is a constituent of 

 normal human urine. The theory that creatinine is derived from 

 the creatine of ingested muscular tissue as well as from the creatine 

 of the muscular tissue of the organism has recently been proven 

 to' be incorrect by Folin, Klercker, and Wolf and Shaffer. Shaffer 

 believes that creatinine is the result of some special process 

 of normal metabolism which takes place to a large extent, if not 

 entirely, in the muscles and further that the amount of such creatin- 



1 Moreigne's reagent is made by combining 20 grams of sodium tungstate, 10 

 grams of phosphoric acid (sp. gr. 1.13) and 100 c.c. of water. Boil this mixture 

 for twenty minutes, add water to make the volume of the solution equivalent 

 to the original volume and acidify with hydrochloric acid. 



