APPENDIX. 421 



Obermayer's Reagent. 1 Add 2-4 grams of ferric chloride to 

 a liter of hydrochloric acid (sp. gr. 1.19). 



Oxalated Plasma. 2 Allow arterial blood to run into an equal 

 volume of 0.2 per cent ammonium oxalate solution. 



Para-dimethylaminobenzaldehyde Solution/"' This solution is 

 made by dissolving 5 grams of para-dimethylaminobenzaldehyde 

 in 100 c.c. of 10 per cent sulphuric acid. 



Para-phenelenediamine Hydrochloride Solution. 4 Two grams 

 dissolved in 100 c.c. of water. 



Phenolphthalein. 5 Dissolve i gram of phenolphthalein in 100 

 c.c. of 95 per cent alcohol. 



Phenylhydrazine Mixture. This mixture is prepared by com- 

 bining i part of phenylhydrazine-hydrochloride and 2 parts of sod- 

 ium acetate by weight. These are thoroughly mixed in a mortar. 



Phenylhydrazine- Acetate Solution. 7 This solution is prepared 

 by mixing i volume of glacial acetic acid, i volume of water and 

 2 volumes of phenylhydrazine (the base). 



Purdy's Solution. 8 Purdy's solution has the following com- 

 position : 



Cupric sulphate 4-752 grams. 



Potassium hydroxide 23.5 grams. , 



Ammonia (U. S. P., sp. gr. 0.9) 35O.O c.c. 



Glycerol 38.0 c.c. 



Distilled water, to make total volume i liter. 



Roberts' Reagent. 9 Mix i volume of concentrated nitric acid 

 and 5 volumes of a saturated solution of magnesium sulphate. 



Rosenheim's lodo-Potassium Iodide Solution. 10 Dissolve 2 

 grams of iodine and 6 grams of potassium iodide in 100 c.c. of 

 water. 



Salted Plasma. 11 Allow arterial blood to run into an equal vol- 



I Obermayer's test, page 281. 



" Experiments on blood plasma, page 201. 



3 Herter's para-dimethylaminobenzaldehyde reaction, page 170. 



4 Detection of hydrogen peroxide, page 226. 



5 Topfer's method, page 413. 



6 Phenylhydrazine reaction, pages 24 and 306. 



7 Phenylhydrazine reaction, pages 24 and 306. 



8 Purdy's method, page 370. 



9 Roberts' ring test, pages 97 and 315. 



10 Rosenheim's periodide test, page 252. 



II Experiments on blood plasma, page 201. 



