TECHNIQUE OF THE COMPLEMENT FIXATION METHOD 



Fleming, Lancet, 1909, 4474. 



Stern, Zeitschr. f. Jmmunitatsforschung, 1909, Bd. I. 



Bauer, Deutsche Med. Wochenschr., 1909, No. 10. 



IV. Serum Diagnosis of Echinococcus Disease. 



The technique of this reaction is practically the same as described for 

 the Wassermann test. 



As antigen the cystic fluid of the human being or sheep is employed. 

 The latter according to Weinberg is preferable, as human hydatid fluid 

 sometimes reacts with normal serum. 



The following is Weinberg's outline for performing the test: 



Bauer's modification as employed for the Wassermann test can also be 

 employed here. 



V. Serum Diagnosis of Other Diseases. 



The method of complement fixation can be employed in most diseases 

 where an antigen can be made of the specific etiological agents, and where 

 antibodies against this antigen have been formed in the serum of the 

 infected subjects. The relative importance of the reaction in a given 



