BACTERIOLYSIS AND ALLIED PHENOMENA 145 



haemolysis occurred. Evidently it had been removed by the first 

 addition of corpuscles. 



Red corpuscles, therefore, have a combining affinity for ambo- 

 ceptor, and in further experiments Ehrlich showed that this 

 combination takes place at ordinary temperatures or at low ones, 

 down to o C. Expressed in the language of the side-chain theory, 

 amboceptor has a haptophore group with a combining affinity 

 for the receptors of the corpuscle, bacterium, etc., with which it 



'*'** 



* t ~ 



FIG. 29. NORMAL GOAT SERUM (COMPLEMENT), PLUS SHEEP'S CORPUSCLES. 



No combination. This is shown as follows : The mixture is centrifugalized, 

 and to the corpuscles heated immune serum (amboceptor) was added 

 (Fig. 30), whilst to the supernatant fluid corpuscles and heated serum 

 were added (Fig. 31). 



FIG. 30. THE CORPUSCLES FROM THE PREVIOUS EXPERIMENT INCUBATED 



WITH HEATED IMMUNE SERUM. 



No solution, showing that no complement had been abstracted in combination 



with them. 



unites. We shall see reasons for believing that it may have a 

 second haptophore group, and shall distinguish this first by the 

 name of cytophile haptophore. 



Ehrlich now investigated the behaviour of the second substance 

 the complement with the red corpuscles by a precisely similar 

 method, and found that the two had no power of entering into 

 combination. Thus, normal goat's blood (containing complement) 

 was added to sheep's corpuscles, and the mixture centrifugalized. 

 To the corpuscles heated immune serum (amboceptor) was added, 

 but there was no haemolysis. Again, to the supernatant fluid 

 sheep's corpuscles and heated serum were added, and haemolysis 



10 



