532 APPENDIX. 



On the addition of a salt of silver to the acidulated urine 

 there was no precipitation of chloride of silver, but when 

 added to neutralized urine a coffee-coloured tint was developed, 

 indicative of uroxanthin. 



On the addition of a salt of baryta there was a slight violet- 

 colour precipitate of sulphate of baryta. 



On evaporating the urine there was left a residue of a dark- 

 blue colour, and bright blue spots were observed on the edges 

 of the capsule. It was washed with water, in order to sepa- 

 rate the urea, and then extracted with cold spirit of 0-830, 

 which dissolved the urrhodin, and formed a carmine solution. 

 On boiling the residue with spirit, a solution of uroglaucin 

 was obtained, which, on cooling, formed beautiful ultramarine- 

 blue crystals. 



The amount of urea was very small, and there was no uric 

 acid or chloride of sodium; on the other hand, there was 

 a large quantity of carbonate of ammonia, and a moderate 

 amount of albumen. The earthy phosphates, phosphate of 

 soda, and sulphate of potash were present in very diminished 

 quantities. Uroglaucin and urrhodin were present to a large 

 amount. 



The urine passed on the following morning (amounting to a 

 little above two ounces) was submitted to analysis. It was of 

 a* bottle-green colour, turbid, and deposited a sediment which, 

 when examined under the microscope, was found to contain 

 crystals of uroglaucin, and indeed all the constituents noticed 

 the preceding day. 



The urine was strongly alkaline, emitting an urinous ammo- 

 niacal odour. The specific gravity was 1013, and the reactions, 

 with nitric acid, alcohol, &c., the same as before. There was, 

 however, a larger amount of albumen. Uroglaucin and urrho- 

 din were likewise present in abundance. 



The urine contained in 1000 parts : 



Water . . . . 971-20 



Solid constituents . . . 28-80 



Urea . . . . 3-8! 

 Uric acid . . . .no trace 



Fixed salts .... 3-80 



Uroglaucin, urrhodin, uroxanthin, extractive "1 14 .o 



matters, and carbonate of ammonia J 



Albumen . . 9-89 



