LEYDENIA GEMMIPARA 49 



pole and the flagellispores swarm out of the shell. 1 The biflagellate 

 swarm-spores, or gametes, copulate in pairs and apparently the indi- 

 viduals of the pairs of gametes arise .from different mother organisms. 

 The zygote secretes a thick covering which soon 

 becomes brown and rough. These zygote cysts 

 or resistant spores must now pass from the in- 

 testine of an animal in order to complete their 

 development. The escape of the cyst contents 

 does not always take place in the intestine ; 

 often it does not occur until after defaecaticn. 

 These shell-less individuals (amoebula) soon 

 become invested with a shell. But in the on the left the old capsule, 

 alkaline intestinal contents, shell formation may kowsld.) 

 proceed even while the organism is in the in- 

 testine, and multiplication may take place. 



Schaudinn's further communication was of special interest ; it was 

 to the effect that Chlamydophrys was related to 



Leydenia gemmipara, Schaudinn, 1896. 



In the fluid removed by puncture from two patients suffering 

 from ascites in the first medical clinic in Berlin, cellular bodies 

 with spontaneous movement were found, which Leyden and 

 Schaudinn regard as distinct organisms. They remained alive 

 without the use of the warm stage for four or five hours, the 

 external temperature being 24 to 25 C. In a quiescent condition 

 they were of a spherical or irregular polygonal form. Their surface was 

 rarely smooth, being beset with protuberances arid excrescences (fig. 15). 

 The substance of the body was thickly permeated with light refractile 

 granules with a yellowish shimmer. The hyaline ectoplasm was 

 rarely seen distinctly. All sizes from 3 p to 36 //, in diameter were 

 observed. The movements were rather sluggish, the ectoplasm in 

 the meantime appearing in the form of one or several lamellae, in 

 which also strings of the granular endoplasm occurred, and frequently 

 protruded over the border of the hyaline pseudopodia. The tendency 

 for the joining of several individuals by means of their pseudopodia 

 was so marked that associations ensued similar to those known in 

 free-living Rhizopoda. 



The cytoplasm enclosed blood corpuscles as well as numerous 

 vacuoles, one of which pulsated slowly about every quarter of an 

 hour. A vesicular nucleus the diameter of which was about equal 

 to one-fifth of the body was present. 



1 Schaudinn (1903), Arb. a. d. Kaiserl. Gesundh., xix, p. 547. 



