io6 



THE ANIMAL PARASITES OF MAN 



There is sometimes a very short, slightly curved filament to be seen,, 

 which may be a rhizoplast. Multiplication takes place by binary or 

 multiple fission. The presence of the parasite used to be demonstrated 

 by splenic or hepatic puncture ; nowadays it can be demonstrated in. 

 peripheral blood, e.g. t of the finger, or by culture of infected blood. 



FlG. 50. Leishmania donovani. z, Free forms, each with nucleus and rod -shaped Hepharo- 

 plast (after Christophers) ; 2, endothelial cell and leucocytes containing parasites (after 

 Christophers) ; j, capillary in the liver showing endothelial cells containing parasites (after 

 Christophers) ; ^, two parasites escaping from a leucocyte in the alimentary canal of the bug 

 (after Patton) ; 5, further development in bug (after Patton) ; 6, young flagellate forms in bug 

 (after Patton) ; 7-//, culture forms (after Leishman) ; 7, <?, 9, show development of flagellum. 



L. donovani can be cultivated in citrated splenic blood, under 

 aerobic conditions, at 22 to 25 C. This was first accomplished 

 by Rogers (1904). It is not so easily culturable as L. infantum on 

 the Novy-MacNeal-Nicolle medium. 1 L. donovani is inoculable with 



1 For the composition of this medium, see Appendix. 



