CONTROL OF THE PUBLIC MILK SUPPLY 413 



54. The proper designated officers of the American Association of Medical 

 Milk Commissions should receive copies of reports of all of the annual, semiannual, 

 and other official tuberculin tests which are made and keep copies of the same on 

 file and compile them annually for the use of the association. 



55. Disposition of Cows Sick with Diseases Other than Tuberculosis. Cows 

 having rheumatism, leukorrhea, inflammation of the uterus, severe diarrhea, or 

 disease of the udder, or cows that from any other cause may be a menace to the 

 herd shall be removed from the herd, placed in a building separate from that 

 which may be used for the isolation of cows with tuberculosis, unless such build- 

 ing has been properly disinfected since it was last used for this purpose. The 

 milk from such cows shall not be used, nor shall the cows be restored to the herd 

 until permission has been given by the veterinary inspector after a careful phys- 

 ical examination. 



56. Notification of Veterinary Inspector. In the event of the occurrence of 

 any of the diseases just described between the visits of the veterinary inspector, 

 or if at any time a number of cows become sick at one time in such a way as 

 to suggest the outbreak of a contagious disease or poisoning, it shall be the duty 

 of the dairyman to withdraw such sickened cattle from the herd, to destroy 

 their milk, and to notify the veterinary inspector by telegraph or telephone 

 immediately. 



57. Emaciated Cows. Cows that are emaciated from chronic diseases or from 

 any cause that in the opinion of the veterinary inspector may endanger the qual- 

 ty of the milk, shall be removed from the herd. 



BACTERIOLOGICAL STANDARDS 



58. Bacterial Counts. Certified milk shall contain less than 10,000 bacteria 

 per cubic centimeter when delivered. In case a count exceeding 10,000 bacteria 

 per cubic centimeter is found, daily counts shall be made, and if normal counts 

 are not restored within 10 days the certificate shall be suspended. 



59. Bacterial counts shall be made at least once a week. 



60. Collection of Samples. The samples to 'be examined shall be obtained 

 from milk as offered for sale and shall be taken by a representative of the milk 

 commission. The samples shall be received in the original packages, in properly 

 iced containers, and they shall be so kept until examined, so as to limit as far as 

 possible changes in their bacterial content. 



61. For the purpose of ascertaining the temperature, a separate original pack- 

 age shall be used, and the temperature taken at the time of collecting the sample, 

 using for the purpose a standardized thermometer graduated in the centigrade 

 scale. 



62. Interval between Milking and Plating. The examinations shall be made 

 as soon after collection of the samples as possible, and in no case shall the interval 

 between milking and plating the samples be longer than 40 hr. 



63. Plating. The packages shall be opened with aseptic - precautions after 

 the milk has been thoroughly mixed by vigorously reversing and shaking the 

 container 25 times. 



64. Two plates at least shall be made for each sample of milk, and there 

 shall also be made a control of each lot of medium and apparatus used at each 

 testing. The plates shall be grown at 37C. for 48 hr. 



