QUALITATIVE BACTERIOLOGICAL ANALYSIS. 71 



The B. indigoferus described by Zimmermann resembles the 

 above organisms, but is said to form spores. 



GROUP VIII. 



This group includes the B. albus of Eisenberg, B. ubiquitus 

 of Jordan, B. aquatilis of Lustig, and B. umbilicatus of Zimmer- 

 mann. 



B. Albus. 



Eisenberg describes this organism as a small motile bacillus 

 which does not grow at 37 C. In gelatine plates the colonies 

 are round and have a pin-head appearance ; the gelatine is not 

 liquefied. In gelatine-stab there is a slow growth ; on the sur- 

 face it forms a pin-head appearance. On agar it produces a 

 milk-white layer. On potato there is a dirty yellowish-white 

 growth limited to the line of inoculation. 



I have found in water an organism corresponding to the above 

 description, which also failed to coagulate milk, and did not 

 produce gas in glucose media, indol in peptone- water, nor reduce 

 nitrates. 



The B. aquatilis of Lustig resembles the B. albus in most of 

 its cultures, but it reduces nitrates to nitrites. 



B. Ubiquitus. 



This organism was isolated by Jordan from Lawrence sewage 

 and stated to be abundant everywhere. It is a small, short, 

 plump bacillus closely resembling a micrococcus. It is non- 

 motile, and grows as well at 37 C. as at 22 C. In gelatine 

 plates the colonies at first are small, round, and of a yellowish 

 tinge; in two days they form glistening white projections 

 resembling drops of milk. Under a low power the colonies 

 have a smooth edge and granular contents. In gelatine-stab 

 there is a good growth both on the surface and along the line 

 of inoculation ; the whole growth resembles a nail. At first the 

 colour of the growth is a lustrous porcelain white; later, it is a 

 dull brownish-grey. On agar there is a whitish-grey growth 

 having a somewhat metallic lustre. On potato there is a white 

 shining growth limited to the line of inoculation. Milk is 

 quickly coagulated with a strong acid reaction. In broth there 



