QUALITATIVE BACTERIOLOGICAL ANALYSIS. Ill 



B. cadaveris, which resembles the B. enteritidis sporogenes, in 

 that it is an obligatory anaerobe. It produces gas in glucose- 

 agar without causing liquefaction, but liquefies glucose-gelatine 

 and solidified blood serum ; it forms drum-sticks and terminal 

 spores, and is conspicuously motile. Striking differences 

 between the two organisms are seen when anaerobic cultures 

 are made on the slanting surface of ordinary agar or on a 

 surface anaerobic plate culture of agar. It is then found that 

 the B. cadaveris forms angular dark granular colonies, from 

 which extend numerous filamentous processes, which later on, 

 as incubation proceeds, form a more or less densely reticulated 

 mass of considerable extent ; at the same time this dark 

 granular material is embedded on a lobulated, finely granular, 

 translucent, filmy basis. In some cultures the filmy basis is 

 absent altogether. On the other hand the colonies of 

 B. enteritidis are circular discs, and there is never the reticulated 

 expansion from the centre, which is seen in the colonies of the 

 B. cadaveris. The B. cadaveris also forms spores on agar and 

 in milk, which is never the case with B. enteritidis. The milk 

 culture of B. cadaveris also has a putrid odour, and its reaction 

 is amphoteric , or perhaps a little alkaline. " The milk cultures, 

 liquefied gelatine cultures, agar cultures and liquefied serum 

 cultures of the B. cadaveris are without pathogenic effect on 

 guinea-pigs ; as much as 2 and 3 c.c. of a liquefied 

 sugar-gelatine, or of a milk culture, can be injected without 

 physiological effect." According to Klein, the B. enteritidis can 

 be distinguished from the bacillus of malignant oedema, &c., by 

 the following points : 



B. Enteritidis Sporogenes. 



(a) It is thicker ; (b) spores at the end of the bacillus ; (c) 

 flagella chiefly in a bundle at the end of the bacillus; 

 (d) gangrenous condition of the tissue at site of injection in 

 a guinea-pig ; (e) butyric acid smell and acid reaction of milk 

 culture, which does not contain spores ; (f ) stains with Gram ; 

 (g) gelatine-stab has not lateral off-shoots. 



Bacillus of Malignant (Edema, 

 (a) Thinner ; (b) spores in the middle of the bacillus ; 



