QUALITATIVE BACTERIOLOGICAL ANALYSIS. 117 



Streptococcus B. 



Gelatine Plates. Small circular colonies, with clear sharp 

 edges, thicker in the centre than at the periphery ; under a low 

 power granular, but no streamers observed. Gelatine was not 

 liquefied. 



Agar -slope. The growth consisted of numerous small isolated 

 circular colonies. 



Gelatine-stab. On the surface a very small circumscribed 

 film, and along the stab a fine growth consisting of small 

 colonies massed together. 



Gelatine-stab at 37 C. After twenty-four hours incubation 

 at 37 C, the tube was nearly clear with a thick deposit at the 

 bottom. 



Glucose-gelatine. No gas formation. 



Milk. Coagulated after forty-eight hours incubation at 

 37 C. 



Litmus-whey. After twenty-four hours incubation at 37 C. 



the acidity equalled 3'3 per cent. -^ alkali ; after seven days 



incubation the acidity equalled 1 3 per cent. -^ alkali. 



Peptone and Salt Solution. No formation of indol. 



Broth. A diffused growth with a marked deposit at the 

 bottom. 



Potato. Moist, glistening surface, growth hardly apparent. 



Staining 1 Reactions. Stains with Gram's method. 



Microscopical Characters. Twenty-four hours broth culture 

 showed chains consisting of seven to eleven cocci. 



Streptococcus C. 



Gelatine Plates. Small circular colonies with clear, sharp 

 edges, thicker at the centre than the periphery ; no streamers 

 observed ; gelatine not liquefied. 



Agar-slope. Very small circular colonies, uniting below to 

 form a thin greyish-white growth. 



Gelatine-stab. On the surface a very small circumscribed 

 film, and along the stab a very fine growth, consisting of small 

 colonies massed together. 



