118 BACTERIOLOGICAL EXAMINATION OF WATER. 



Gelatine-stab at 37 C. Diffused growth through the tube. 



Glucose-gelatine. No gas formation. 



Milk. Coagulated in forty-eight hours. 



Litmus-whey. After twenty-four hours incubation at 37 C. 



the acidity equalled 5 per cent. ~ alkali. After seven days 



incubation at 37 C. the acidity equalled 14 per cent. ^ alkali. 



Peptone and Salt Solution. No formation of indol. 



Broth. A diffused growth with deposit at the bottom of the 

 cube ; after ten days growth the tube was still turbid. 



Potato. Glistening transparent growth. 



Staining Reactions. Stains with Gram's method. 



Microscopical Characters. Twenty-four hours broth culture 

 showed very long chains, consisting of at least thirty cocci. 

 NOTE. Streptococcus C. was only distinguished from B. by the 

 small size of its colonies on agar and the extreme length 

 of the chains in the broth culture. 



Streptococcus D. 



This culture very closely resembled streptococcus C. in the 

 size of its colonies on agar, its re-actions in milk, litmus-whey, 

 and glucose-gelatine, and on potato. It, however, only pro- 

 duced very short chains in a twenty-four hours broth culture, 

 and gave an indol reaction in peptone. 



Streptococcus E. 



Gelatine Plates. Colonies circular, larger and more opaque 

 the above varieties, consisting of very small cocci. 



Agar-slope. A thick, white, opaque growth ; at the margin, 

 large, white, and opaque, discrete colonies like those on the 

 gelatine plates. 



Gelatine-stab. Thick, white, opaque growth on the surface 

 and along the stab. After fourteen days at 22 C. gelatine 

 slowly liquefied. 



Gelatine-stab at 37 C. Tube clear, with a deposit at the 

 bottom after twenty-four hours incubation. 



Broth. At the end of twenty-four hours at 37 C. faintly 



