QUALITATIVE BACTERIOLOGICAL ANALYSIS. 121 



Streptococcus, J 10 . Surface- gelatine Plates. Colonies 

 minute, circular, transparent, yellowish -grey in colour, showing 

 very faint granulation. The edge is clean, there are no loops 

 visible, no liquefaction. Surface-agar Plates. The colonies 

 are darker, less transparent, and the granulation, which is wavy 

 in character, is much more marked. The colonies are usually 

 more or less circular in shape ; the edge is usually clean. Broth. 

 Diffuse but not very abundant cloudiness in twenty-four 

 hours. At foot of tube a white and somewhat viscous deposit. 

 Litmus-mill;. In three days distinct acidity but no clot; no 

 clot sixth day. Gelatine-slope. Minute, circular, transparent 

 colonies. Morphology. Stains with Gram. Cocci chiefly in 

 adherent masses, but also separate chains. 



Streptococcus K n . Surf ace- gelatine Plates. Minute, 

 transparent, yellowish-grey, nucleated, faintly granular colonies 

 with clean edge ; no liquefaction. Surface-agar Plates. The 

 colonies are darker and less transparent and show a wavy and 

 coarse granulation ; some of the colonies are quite circular with 

 clean or only very slightly frayed edge. Other colonies are 

 irregular in shape with a broken-up edge. Broth. Remains 

 quite clear, on the sides of the tube and at the foot fluffy white 

 masses are to be seen, which are of a somewhat viscous nature. 

 Litmus-milk. Practically no change visible after eight days at 

 37 C. Gelatine-slope. Minute circular transparent colonies. 

 Morphology. Stains with Gram. Cocci chiefly in masses, many 

 of them of large size and of irregular shape ; ? also chains. 



Streptococcus L 12 . This appeared to be practically the 

 same as K n , but it grew more slowly. 



Streptococcus C 3 . Surface-gelatine Plates. Circular, trans- 

 parent, minute, faintly granular, yellowish- white colonies. No 

 loops of cocci visible at the edge ; of the colonies; no liquefac- 

 tion. Surface-cigar Plates. More or less circular minute 

 colonies of yellowish-white colour, faintly granular, with un- 

 broken edge. Broth. Only slight diffuse cloudiness, not much 

 deposit at the foot of the tube. Litmus-milk. Very feeble 

 acidity, no clot after fifteen days at 37 C. Gelatine-slope. 

 Minute greyish-white circular colonies, nearly transparent. 

 Morphology. Stains with Gram. Chains of cocci of medium 

 length, not matted in masses. 



