QUALITATIVE BACTERIOLOGICAL ANALYSIS. 159 



There was no growth on potato, and milk was rendered viscous. 

 It was a large, non-motile bacillus, with an oval spore towards 

 one end. It stained with Gram. 



Bacillus III. Isolated from soil ; was characterised by small 

 pin-head colonies. There was a viscous deposit and a stringy 

 pellicle in broth, and a positive indol reaction was obtained. 

 On potato there was a cream-coloured growth, and milk was 

 converted into a solid curd. In was a large, non-motile bacillus, 

 with a terminal spore, and stained with Gram. 



Bacillus IV. From ensilage; produced dull, white spherical 

 colonies. There was an indol reaction in broth, and milk was 

 converted into a solid curd. There was no growth on potato. 

 It was a large bacillus, with oscillatory movements. It stained 

 with Gram, and contained a small oval spore towards the 

 slightly swollen end. 



Bacillus V. From Thames mud ; gave rise to spherical, 

 yellowish-white colonies, with regular margin. Under a low 

 power the centre appeared dark-brown and finely granular ; the 

 outer zone was pale yellow, coarsely granular, with ridged 

 markings. Its reactions in broth, milk, and on potato were 

 the same as No. IV. The bacillus in form was more slender 

 than No. IV., and did riot stain with Gram. 



Bacillus VL Was obtained from Plymouth sea-water, and 

 produced dull, white, feathery colonies. It was a slender, non- 

 motile bacillus with a drum-stick spore. 



Bacillus VII. From soil ; produced round, dry, feathery 

 colonies. There was no perceptible growth in broth. On 

 potato there was a vigorous, moist, brick-dust-coloured growth. 

 Milk was converted into a solid curd. The bacillus was non- 

 motile ; about the size of the anthrax bacillus, and appeared 

 in pairs and short chains. It stained with Gram, and had a 

 large terminal spore. 



Bacillus VIII. From soil ; gave rise to yellowish-white 

 spherical colonies. On agar there was a pale lemon-coloured 

 growth. On potato a moist, cream-coloured growth. There 

 was an indol reaction in broth, and milk was coagulated with 

 separation of whey. It was a large bacillus with oscillatory 

 movement, and contained a large oval central spore. 



Bacillus IX. From soil ; produced spherical colonies, with a 



