160 BACTERIOLOGICAL EXAMINATION OF WATER. 



dull ground-glass appearance. On agar and potato there was 

 a white, crusty growth. Milk was coagulated, with separation 

 of whey. An indol reaction was obtained in broth. It was a 

 large, non-motile bacillus with a tendency to long thread 

 formation. It possessed a very small terminal spore, and stained 

 with Gram. 



Bacillus X. From soil; was characterised by dull, white, circular 

 colonies. On potato there was a dry, felt-like, reddish-brown 

 growth. Milk was converted into a solid curd. There was no 

 indol reaction in broth. It was a very large motile bacillus 

 with a large, terminal oval spore. 



Bacillus XI. From soil ; formed round, dull, white colonies 

 with a moist, glistening appearance. On potato there was 

 a moist, brownish-yellow growth. Milk was coagulated with 

 separation of whey. There was an indol reaction in broth. It 

 was a large bacillus, which formed long chains. It stained with 

 Gram and had .a terminal oval spore. 



Bacillus XII. From soil ; produced stellate, yellowish-white 

 colonies. On potato there was a dry, pipe-clay coloured growth. 

 Milk was converted into a solid curd. There was an indol 

 reaction in broth. It was a large, non-motile bacillus with a 

 drum-stick spore. It did not stain with Gram. 



Bacillus XIII. From soil ; produced round, yellowish-white 

 colonies. On potato there was a moist, yellowish-brown growth. 

 Milk was coagulated with separation of whey. In broth an 

 indol reaction was obtained without the addition of a nitrite ! 

 It was a large, non-motile bacillus with square ends. It stained 

 with Gram, and possessed a spore towards the swollen end of the 

 bacillus. 



Bacillus XIV. From soil ; gave rise to a thin surface film over 

 agar, with discrete and circular colonies at the margin. On 

 potato there was a moist, slate-coloured growth. Milk was 

 coagulated, with separation of whey. There was an indol 

 reaction in broth. The organism was motile, formed short 

 slender rods, surrounded by a zoogloea. It stained with Gram 

 and contained a spore in the centre of the bacillus. All the 

 above organisms grew well at 55 to 65 C. on ordinary media. 



Macfadyen and BlaxalFs experiments did not support Rabino- 

 witsch's statement that at low temperature thermophylic bacteria 



