18fi BACTERIOLOGICAL EXAMINATION OF WATER. 



Chamberland bougie, and the filtrate divided amongst a number 

 of small flasks. As a rule, the flasks were kept for from four to 

 .six weeks before being filtered. The flasks containing the 

 filtrate were then incubated to ascertain whether the broth had 

 been completely freed from bacilli. If there was no turbidity 

 after incubation of the filtrate, the flasks were inoculated with 

 different bacilli. It was found that when the B. typhosus was 

 inoculated into flasks containing the toxins of the following 

 bacilli, the results recorded below were obtained : 



In the toxins of : 



Staphylococcus pyogenes aureus, the B. typhosus grew very feebly. 

 Staphylococcus pyogenes albus, the B. typhosus did not grow. 

 Staphylococcus pyogenes foetidus, the B. typhosus did not grow. 

 B. pyocyaneus, the B. typhosus did not grow. 

 B. typhosus, the B. typhosus grew very feebly. 



B. of symptomatic anthrax, the growth of the B. typhosus was delayed. 

 Chicken cholera, the growth of the B. typhosus was very feeble. 

 Pneumonococcus of Friedlander, the growth of the B. typhosus was very 



feeble. 



B. phosphorescens, the B. typhosus did not grow. 

 Spirillum of Asiatic cholera, the B. typhosus grew feebly. 

 Spirillum of Finkler-Prior, the B. typhosus grew well. 

 Spirillum of Miller, the B. typhosus grew very feebly. 

 Spirillum of Deneke, the B. typhosus grew very feebly. 



In flasks containing the toxins of the B. typhosus it was 

 found that the following bacilli grew well, viz., Staphylococcus 

 pyogenes aureus, albus, citreus, and foetidus ; Micrococcus 

 tetragenus ; Micrococcus prodigiosus ; Micrococcus rose us ; 

 Bacillus of blue milk ; Bacillus pyocyaneus ; Bacillus of sympto- 

 matic anthrax ; Bacillus megaterium ; Bacterium phosphorescens ; 

 Pneumonococcus of Friedlander ; Spirillum of Asiatic cholera ; 

 Spirillum of Finkler-Prior; Spirillum of Miller; Spirillum of 

 Deneke. The micro-organism of chicken cholera grew feebly, and 

 the B. typhosus very feebly in typhoid toxins. The influence of 

 the products of B. fluorescens putridus and B. fltiorescens lique- 

 faciens on the B. typhosus have already been described. The 

 influence of the toxins of B. coli on B. typhosus will be fully 

 considered under the section devoted to the experimental 

 researches on the duration of life of the B. typhosus in water 

 and sewage. 



