QUALITATIVE BACTERIOLOGICAL ANALYSIS. 187 



THE RELATION OF THE TYPHOID BACILLUS TO ACIDS AND 

 ALKALIES IN NUTRIENT MEDIA. 



The effects of the presence of acids and alkalies in nutrient 

 media on the development of the B. typhosus have been care- 

 fully investigated by Kitasato. Broth and gelatine media were 

 carefully neutralised, and varying quantities of the substances 

 under examination added. The medium was then inoculated 

 with a culture of the typhoid bacillus. When gelatine was 

 employed " roll cultures " were made, and the development of 

 the colonies of B. typhosus examined in the usual manner. 



The sign + indicates growth, + growth restrained, no growth. 



