194 BACTERIOLOGICAL EXAMINATION OF WATER. 



formed in lactose-gelatine-shake cultures. Milk is not coagu- 

 lated. There is no formation of indol in Witte's peptone and 

 salt solution after seven days incubation at 37 C. The growth 

 on potato varies ; sometimes it is colourless and transparent, 

 like that of B. typhosus, at other times there is a yellowish 

 slimy growth, resembling that produced by B. coli. A growth 

 takes place after twenty hours incubation in both of Proskauer 

 and Capsldi's media. In No. I. the reaction is sometimes acid 

 and sometimes alkaline ; in No. II. the reaction is either neutral 

 or alkaline. The behaviour of the bacillus in litmus-whey is 

 peculiar and very characteristic. It rapidly produces a large 

 quantity of acid ; this is followed later by the formation of 

 alkali, which neutralises the acid, so that the litmus-whey is 

 acid for the first two or three days and then becomes alkaline. 

 In microscopical characters the bacillus is almost identical with 

 B. typhosus. It is not stained by Gramas method. The flagella, 

 as a rule, are fewer than those of B. typhosus, but more numerous 

 than those of B. coli. In its reaction to anti-typhoid serum the 

 B. enteritidis shows less susceptibility to the specific agglutinins. 

 than the B. typhosus. Durham states that B. enteritidis is not 

 agglutinated by anti-typhoid serum diluted 150,000. As a 

 rule, this is true, but on one occasion I obtained a culture which 

 was completely agglutinated by anti-typhoid serum in this 

 dilution. 



The B. enteritidis was found by Karlinski four times in normal 

 faeces, and Germane and Maurea have found bacilli closely 

 resembling it in water. 



The Bacillus Breslaviensis belongs to the Gartner group, and 

 was found by Van Ermenghem and Kansche in outbreaks of 

 meat poisoning in Morseele and Breslau. It is very motile^ 

 possesses from four to twelve flagella, and does not stain with 

 Gram. The colonies are coliform, broth becomes turbid, indol 

 is not produced, and milk is not coagulated. On potato there 

 is a thick yellowish growth. In agar and broth containing 

 grape-sugar, milk-sugar, or cane-sugar, gas is produced, but 

 the quantity is small, except in the media containing grape- 

 sugar. 



The Bacillus Friedebergensis described by Gaffky and Paak, 

 ; ,s also closely allied to Gartner's bacillus. It is about one-third 



