QUALITATIVE BACTERIOLOGICAL ANALYSIS. 235 



pinch-cock every third day, about 10 c.c. of the toxins, 

 excreted by the typhoid bacillus, were allowed to filter 

 through into the Kitasato flask. The amount of fluid filtered 

 from the mantle was replaced by sterile broth. The pro- 

 cedure was continued for two months until the flask was 

 filled nearly to the level of the arm. A loopful was then 

 withdrawn and planted out on agar. The growth which resulted 

 was tested for agglutination with Berne anti-typhoid serum, 

 and sub-cultured in various media. No change was observed in 

 its sensibility to agglutination or in its cultural reactions. The 

 flask containing the mixture of colon bacilli and typhoid toxins 

 was kept at a temperature of 25 C. for a further two months. 

 A loopful was then withdrawn as before, and the growth tested 

 with Berne serum. No increased sensibility to agglutination 

 was noticed. 



It is impossible to imitate by experiment the complex condition 

 of things which exists in the human body suffering from a 

 typhoid infection, so it is not surprising that the agglutinins 

 and toxins in vitro failed to influence the sensibility of the 

 colon bacillus to agglutination. 



