QUALITATIVE BACTERIOLOGICAL ANALYSIS. 



sisting of a slimy, greyish -yellow layer, which rapidly spreads 

 over the potato. 



Peptone and Salt Solution. Asa rule, no indol reaction can 

 be obtained on the addition of sulphuric acid, as, though it 

 produces indol, nitrites are not manufactured in sufficient 

 quantity to give the cholera-red reaction. In some cases more 

 nitrites are produced, and the reaction is then developed slowly 

 with a faint reddish tint. 



Microscopical Appearances. The organism is a comma-shaped 

 bacillus, slightly thicker in the middle than at the ends ; it 

 is larger than Koch's organism, highly motile, and possesses a 

 single flagellum. 



When injected into the peritoneum of a guinea-pig it causes 

 peritonitis ; and in man, after neutralisation of the acid in the 

 stomach, it causes intestinal disturbance (Metchnikoff*). 



The vibrio. of Tinkler-Prior is easily distinguished from the 

 typical cholera spirillum by its form, rapid liquefaction of 

 gelatine, and failure to give the cholera-red reaction. 



Vibrio Metchnikovi. 



This microbe was discovered by Gamale'ia at Odessa, where 

 it produced epidemic disease amongst fowls. It is of great 

 interest owing to its very close resemblance to Koch's spirillum. 

 An organism closely resembling the vibrio Metchnikovi was 

 isolated by Pfuhl from water. 



Gelatine Plates. The young colonies may have a perfectly 

 smooth outline, though sometimes the margin is distinctly 

 crenated like that of the cholera colonies. The colonies 

 generally grow more quickly, and liquefy more rapidly, than 

 those of Koch's vibrio ; in forty-eight hours the margin is often 

 very irregular and torn, and resembles Finkler-Prior's colonies. 



Gelatine-stab. The appearances closely resemble those pro- 

 duced by Koch's vibrio ; liquefaction, however, occurs more 

 rapidly ; the rate seems to be intermediate between Finkler- 

 Prior's and Koch's organisms. 



Agar-slope. A greyish white, moist growth. 



Potato. A raised, dirty-brownish growth. 



Broth. Diffuse growth in twenty-four hours ; a surface 

 pellicle is eventually formed. 



