QUALITATIVE BACTERIOLOGICAL ANALYSIS. 24,5 



Gelatine-stab. There is growth on the surface, and lique- 

 faction slowly takes place, forming a basin- shaped depression. 

 There is very little growth along the stab. 



Agar. Forms a greyish-white growth, not characteristic. 



Broth. It is said not to grow in broth at 37 C. ; and at 

 21 to 22 C. the growth is extremely slow. 



Peptone and Salt Solution. The cholera-red reaction is not 

 obtained on adding a few drops of pure sulphuric acid. 



Potato. No growth takes place at 22 C. or at 37 C. 



Microscopical Appearances. It is a highly motile vibrio 

 exactly resembling the cholera spirillum. It possesses one 



flagellum. 



Vibrio Berolinensis. 



This microbe was isolated by Neisser and Glinther from 

 Berlin pipe-water. The specimen of water had been designedly 

 infected with true cholera bacilli some time before, so there was 

 a possibility that the Vibrio berolinensis was a variety of the 

 cholera spirillum, modified by existence in water. 



Gelatine Plates. It grows extremely slowly, and in twenty- 

 four hours the colonies are only visible with the aid of the 

 microscope, and are round and finely granular. Even in forty- 

 eight hours the colonies are not visible to the naked eye. 



Gelatine-stab. Growth extremely slow, resembles that of the 

 cholera vibrio. 



Agar. White growth, not characteristic. 



Potato. Growth like the cholera vibrio. 



Milk. Unchanged . 



Broth. Diffuse growth. 



Peptone and Salt Solution. Cholera-red reaction obtained. 



Microscopical Characters. A motile vibrio about the same 

 size as Koch's spirillum ; it posseses one flagellum. It is 

 decolorised by Gram's method, and is pathogenic to guinea- 

 pigs. It does not give Pfeiffer's reaction. 



Vibrio Massowah. 



This vibrio was isolated from the stools of a sick person 

 during a small epidemic of cholera. It was at first accepted 

 as a true cholera spirillum, but later researches showed that 

 there were differences between the two organisms. The Vibrio 



