i>48 BACTERIOLOGICAL EXAMINATION OF WATER. 



growth. In peptone and gelatine-stab the appearances were the 

 same as No. 29. The colonies were also very similar to those of 

 17 A ; those in the depth, however, had an irregular margin and 

 were coarsely granular. 



Vibrio No. 34 was rather larger than the above, and possessed 

 one long flagellum. On agar, in gelatine-stab and peptone, the 

 growths were the same as No. 29. In gelatine plates small dry 

 hollows appeared, containing at the bottom dry white colonies, 

 which, under a low power, exactly resembled No. I?A. 



Vibrio No. 4& was rather small, curved, with rounded ends 

 and highly motile. On agar, in peptone, and in gelatine the 

 growths resembled No. 29. In gelatine plates dry hollows 

 which contained thin grey colonies were seen. The colonies in the 

 depth were sharply outlined greenish-yellow, and finely granular. 



Vibrio No. 51 closely resembled No. 45 both in form and in 

 the appearance of the colonies in gelatine plates. In peptone 

 and gelatine-stab the growths were the same as I?A. 



Bacillus Choleroides a 



This vibrio Avas isolated by Bujwid from river water. A little 

 peptone was added to some of the water placed in a flask ; after 

 incubation for three days a loopful from the surface plated out 

 in gelatine showed, after three to six days at room-temperature 

 (10-12 R.), very suspicious colonies resembling the cholera 

 spirillum. At a higher temperature, however, the colonies 

 grew out in a more superficial manner, and were broader and 

 did not sink so much into the gelatine. A smell of methyl- 

 merkaptan was observed. Under a low power the colonies had 

 a sharp, regular outline, and a smooth or very finely granular 

 appearance. In gelatine-stab it grew chiefly on the surface, and 

 liquefied only the upper layers. At a temperature of 10 C -12 R. 

 the gelatine was more slowly liquefied, and the air-bubble, 

 so characteristic of the cholera vibrio, was produced. In the 

 depth of the stab there was very little growth. On agar-slope 

 there was a marked growth, with a smell resembling methyl- 

 merkaptan. Broth showed very little growth, and no surface 

 pellicle was formed. There was no indol reaction. Microscopi- 

 cally the vibrio was not so motile as the cholera vibrio, and 

 longer and shorter forms were seen. 



