QUALITATIVE BACTERIOLOGICAL ANALYSIS. 



B. Choleroides j3. 



This organism was isolated by Orlowski, Bujwid's assistant, 

 from a well, in the neighbourhood of which there had been 

 several cases of cholera. It resembled the cholera vibrio more 

 closely than the above, but grew more anaerobically and pro- 

 duced a more marked funnel-shaped liquefaction. 



VIBRIOS ISOLATED FROM WATER BY SANARELLI. 



Sanarelli isolated thirty-two vibrios from water. Eight were 

 found in the Seine below Paris; of these one gave a very 

 marked nitroso-indol reaction, and its biological characters were 

 as follows : 



Morphology. A motile, thin, curved vibrio, often presenting 

 a spiral form. 



Culture on Gelatine. Forms the characteristic bubble of air 

 after twenty-four hours, and liquefies in a typical funnel form. 



Culture in Solution of Peptone-gelatine. Rapid development ; 

 a pellicle forms at the surface in twenty-four hours. 



Culture on Agar. Marked development at 37 C. on agar 

 made with and without broth. 



Culture in Broth. Rapid growth, with formation of a super- 

 ficial pellicle in twenty-four hours. 



Culture on Potato. Brownish circumscribed growth. 



Nitroso-indol Reaction. Very marked in twenty-four hours. 



Indol Reaction. Very marked in twenty-four hours. 



Nine other vibrios were isolated from the upper part of the 

 Seine where it flows through Paris. None of these gave a 

 marked cholera-red reaction ; the colour was either absent or 

 just perceptible after eight days incubation. 



In the effluent from the sewage of Paris after irrigation, 

 vibrios were also found which gave a marked nitroso-indol 

 reaction. The biological characters of these organisms were as 

 follows: 



No. XXV. -Gennevilliers. 



Morphology. A motile vibrio, but longer and thicker than 

 the true cholera microbe. 



Culture on Gelatine. Development along the line of inocula- 

 tion, with funnel-shaped liquefaction at the surface. 



