250 BACTERIOLOGICAL EXAMINATION OF WATER. 



Culture in Solution of Peptone- gelatine. Diffuse growth, with 

 a pellicle at the surface. 



Culture on Agar. Good growth on the agar made without 

 broth ; no growth on the agar containing broth. 



Culture in Broth. Very feeble after twenty-four hours ; later 

 very abundant with a surface pellicle. 



Culture on Potato. Insignificant. 



Nitroso-indol Reaction. Negative after twenty-four hours ; 

 very marked after eight days. 



No. XX VI. G-ennevilliers. 



Morphology. Very thin motile vibrio. 



Culture on Gelatine. Development along the line of inocula- 

 tion, with a small cup-shaped liquefaction at the surface. 



Culture in a Solution of Gelatine-peptone. Abundant growth 

 with formation of a pellicle. 



Culture on Agar. Abundant growth on agar free from 

 broth ; none on the agar made with broth. 



Culture in Broth. Very small after twenty-four hours ; later, 

 more abundant, with formation of a surface pellicle 



Culture on Potato. Insignificant. 



Nitroso-indol Reaction. Negative after twenty-four hours ; 

 very marked after eight days. 



No. XXVII. Gennevilliers. 



Morphology. A motile vibrio, less curved, longer, and a little 

 thicker than the above. 



Culture on Gelatine. Abundant growth, with liquefaction 

 along the line of inoculation and characteristic bubble of air ; 

 after four days typical liquefaction in the form of a funnel. 



Culture on Agar. Very rapid on both forms of agar. 



Culture in Broth. Abundant, with a superficial pellicle after 

 twenty-four hours. 



Culture on Potato. A light grey layer. 



Nitroso-indol Reaction. Slight after twenty-four hours ; verv 

 marked with a scarlet colour after eight days. 



In the water of the Seine at Versailles another vibrio was 

 found closely resembling No. XXVII. It, however, gave a very- 

 marked nitroso-indol reaction after twenty-four hours incubation. 



