10 Animal Micrology 



of this stain already prepared to be used in case the student's 

 preparation is not ready in time 



NOTE. At this point the student should begin chapter Hi 

 in order that no time may be lost. The present chapter may 

 then be completed while the tissues are becoming fixed and 

 hardened. 



14. Picro-Carmine. This stain is perhaps best procured 

 ready made from dealers. If it is desired to make it, consult 

 Appendix B, reagent 63. Quantity necessary, about 50 c.c. 



15. Bordeaux Red. 



Bordeaux red 1.0 gram 



Distilled water 100.0 c.c. 



16. Lyons Blue. 



Absolute alcohol 100.0 c.c. 



Bleu de Lyon 0.3 gram 



17. Eosin.- 



Eosin 0.5 gram 



Alcohol, 95 per cent 100.0 c.c. 



18. Iron Hematoxylin (Heidenhain's). Two solutions are 

 used. They are not to be mixed. 



Solution I. 



Ferric alum (clear violet crystals) .... 2.5 gram 

 Distilled water 100.0 c.c. 



Solution II. 



Hematoxylin 0.5 gram 



Distilled water 100.0 c.c. 



The hematoxylin should ripen (see 13) for some three or 

 four weeks. 



19. Safranin. 



Safranin"O" 1.0 gram 



Absolute alcohol 10.0 c.c. 



Anilin water 90.0 c.c. 



Make the "anilin water" by shaking up 5 c.c. of anilin oil in 

 90 c.c. of distilled water. Filter through a wet filter. Dis- 

 solve the safranin in the anilin water, then add the alcohol. 



