12 



Animal Micrology 



21. Mayer's Albumen Fixative. Chop the white of an egg 

 with scissors and filter it through moist filter paper. It filters 



through very slowly. Add an 

 equal volume of glycerin, and a 

 bit of salycilate of soda (1 gram 

 to 50 c.c.) or thymol to prevent 

 putrefaction. 



22. Celloidin. Put 5 grams of 

 Schering's shredded or granular 

 celloidin into a celloidin bottle (a 

 bottle with glass stopper and 

 ground glass cap) and dissolve it 

 in equal parts of absolute alcohol 

 and ether (see 4). Add only suf- 

 ficient fluid (about 100 c.c.) to 

 make a thick, syrupy mass. In a 

 second celloidin bottle make a thin 

 solution by taking about one-third 

 of the original solution and dilut- 

 ing it with its own volume of the ether-alcohol. Label the 

 bottles thick and thin celloidin, respectively. 



23. Paraffin. In one of the cups of a warm paraffin oven 

 (Fig. 24, 25, or 26), put 75 grams of paraffin, melting at about 

 53 C. The bath should be kept 

 at a temperature of some two 

 degrees above the melting-point 

 of the paraffin. A supply of softer 

 and of harder paraffin (e. g., melt- 

 ing at 43 and 60 C.) should also 

 be at hand. 



Other Reagents. Provide your- 

 self with 200 c.c. of xylol, 25 c.c. 

 of clove oil, 25 c.c. of glacial acetic 

 acid, 50 c.c. of cedar-wood oil, 75 

 c.c. of chloroform, 30 c.c. of gly- 

 cerin and 250 c.c. of absolute 



FIG. 25. Simple Water-Bath. 



This is a useful bath for individual 

 workers. It is provided with imbedding- 

 cups, infiltration vials, a shelf for watch- 

 glass imbedding or for warming instru- 

 ments, and tubulatures for gas regulator 

 and thermometer. 



FIG. 26. Imbedding-Table. 



There should be two rectangular 

 boxes (about 3X3X16 cm.) to contain 

 paraffin. When in use the boxes are so 

 placed on the imbedding-table that the 

 paraffin in one end remains melted ; in 

 the other, solid. Regulate the tempera- 

 ture by placing the flame at the proper 

 distance under the acute angle of the 

 table. It is best, when gas is used, al- 

 ways to turn on the gas completely and 

 then regulate the height of the flame by 

 means of a clamp on the rubber tubing 

 which conducts gas to the burner. 



