Chapter VIII: The Freezing Method 



69 



9. Transfer the sections through the successive grades of 

 alcohol (decolorizing with acid alcohol if necessary) up to absolute 

 alcohol, leaving them two minutes in each, after which remove 

 them to carbol-xylol for 5 minutes or until clear. If desired, 

 stain with eosin (30 to 60 seconds) after 70 per cent, alcohol. 



10. Remove one or two of the best to a slide, drain off the 

 excess of carbol-xylol, add a few drops of balsam and a cover-glass 

 of suitable size, and label. 



11. Remove the sections reserved in step 7 to a test-tube con- 

 taining a small amount of water, and shake the test- tube vigorously 



FIG. 34. Ether or Rhigolene Freezing Attachment. 



for a minute or two. This removes the lymphocytes from the 

 sections, and exposes the reticular connective tissue so that it may 

 be examined. Dehydrate the sections and mount in balsam. 



MEMORANDA 



1. Fresh Tissues Are Frequently Sectioned by the freezing method. The 

 tissue may be transferred directly to the disk of a microtome without 

 previous imbedding, and sectioned after freezing. This affords a ready 



