166 Animal Micrology 



If the tissues are to remain several days or weeks in alcohol, the 

 alcohol will gradually extract it. If they are to be used within a 

 few days, however, it is necessary to remove the excess of subli- 

 mate by adding tincture of iodine to the 70 per cent, alcohol. 

 Sufficient of the tincture is added to give the alcohol a port- wine 

 color ; as often as the color disappears the iodine must be renewed. 

 After from 12 to 48 hours of this treatment, the iodine color 

 persists and the object should then be transferred to fresh 70 or 

 80 per cent, which must be renewed until it no longer extracts 

 iodine from the specimen. 



(2) In handling corrosive sublimate, a glass or horn spoon 

 should be used instead of a metal instrument, because it corrodes 

 metal. 



(3) Use distilled water, not tap water, in making an aqueous 

 solution. 



14. Corrosive Sublimate and Acetic Acid. 



Corrosive sublimate, saturated aqueous solution 



100 parts 



Glacial acetic acid 5 to 10 parts 



This is an excellent reagent for embryonic tissues and for 

 organs which do riot contain a very great amount of connective 

 tissue. See remarks under 13. 



15. Corrosive Sublimate, Nitric Acid Mixture (Gilson's mercuro- 

 nitric mixture). See chap, i, reagent 7 and chap, iii, 2. 



Erlicki's Fluid, see 7. 



16. Ether Alcohol. Equal parts of sulphuric ether and abso- 

 lute alcohol. 



Flemming's Solution, see 11. 



17. Formalin. See chapter i, reagent 6 and chap, iii, 4. 

 It should be borne in mind that formalin is a reducing agent and 

 will rapidly decompose such reagents as osmic acid or chromic 

 acid if mixed with them. 



18. Formalin, Alcohol, and Acetic Acid (Lavdowsky's mix- 

 ture ) . 



Formalin, commercial 10 parts 



Alcohol, 95 per cent 50 parts 



Glacial acetic acid 2 parts 



Distilled water 40 parts 



