188 Animal Micrology 



this fluid, but tissues may be made permanent by neutralizing the 

 alkali by means of acetic acid. 



77. Gage's Formaldehyde Dissociator. See chap, i, reagent 10. 

 For method of using see chap, x, A. 



78. Hertwig's Macerating Fluid. See chap, x, C. 



79. Landois' Solution. 



Neutral ammonium chromate, saturated 



solution 5 grams 



Potassium phosphate, saturated solution . 5 grams 



Sodium sulphate, saturated solution . . 5 grams 



Distilled water 100 c.c. 



This solution is valuable for the central nervous system. Small 

 pieces of tissue are placed in the fluid for 1 to 5 days. For stain- 

 ing after maceration, it is recommended that the material be 

 placed for 24 hours in ammonia carmine diluted with one volume 

 of the macerating fluid. 



80. MacCallum's Macerating Fluid. 



Nitric acid 1 part 



Glycerin 2 parts 



Water . . . 2 parts 



This fluid is recommended for heart muscle of adults or 

 embryos. Hearts should remain in it from 8 hours to 3 days, 

 according to size. The method is valuable for showing the 

 arrangement of cardiac muscle fibers. 



81. Ranvier's One-Third Alcohol. This is one of the common- 

 est as well as one of the best macerating fluids. It is simply a 

 30 per cent, alcohol. Epithelia will macerate in it sufficiently 

 in 24 hours. A still weaker alcohol (20 to 25 per cent.) is used 

 for isolating the nerve fibers of the retina. 



82. Schiefferdecker's Fluid. 



Methyl alcohol 5 c.c. 



Glycerin ' 50 c.c. 



Distilled water 100 c.c. 



This fluid is used for the retina and central nervous system. 

 It should be prepared fresh before using and tissues must remain 

 in it for several days. 



83. Sodium Chloride. A 10 per cent, solution of sodium chlo- 

 ride is excellent for tendon, etc. It dissolves the cement sub- 





