the thickening at the heel. Free the muscle up 

 to its origin from the femur, taking care not to 

 harm the branch of the nerve which enters the 

 muscle on its posterior surface near the knee. 

 Cut through the tibia about one 

 centimetre from the knee-joint. 

 Clear away the muscles of the 

 thigh from the lower end of the 

 femur, avoiding the sciatic nerve. 

 Cut through the femur about 

 its middle. (Fig. 2.) Lay the 

 sciatic nerve for safety along the 

 gastrocnemius muscle. Fasten 



the lower fragment of the femur Fi s- 



cle preparation; gas- 



in the jaWS Of the mUSCle Clamp, trocnemius muscle 



. . and sciatic nerve. F, 



Let the whole nerve rest with- end of femur ; N, sci- 



out stretching on the adjustable 



plate or nerve-holder, the filter 



paper covering which should be to femur (Handbook 



for the Physiological 



moistened with normal saline Laboratory). 

 solution (0.6 per cent NaCl). 

 Take care that the nerve does not dry between 

 the nerve-holder and the muscle. (Fig. 3.) The 

 filter paper should reach from the nerve-holder 

 to the muscle. 



Preliminary Considerations regarding Energy, 

 Stimulation, and Irritability. Pinch^ the muscle 

 sharply with the forceps. 



