FERMENTATION 211. 



pose an extract of lipase may be used, first, to 

 split a neutral fat (or glycerol ester) into its con- 

 stituent fatty acid and alcohol (glycerine is a 

 trihydric alcohol), and second, to form a neutral' 

 fat from fatty acid and alcohol. 



Extraction of Lipase. From Pancreas. Re-- 

 move the pancreas of the pig within thirty min- 

 utes after the death of the animal. Dissect off 

 as much of the fat as possible. Reduce the pan- 

 creas to a fine pulp in a mortar with coarse well- 

 washed white sand. Extract- the lipase with a 

 little water or glycerine. 



From Liver., Remove the liver of the pig 

 within thirty minutes of the death of the animal. 

 Reduce 50 gms. to a fine pulp in. a mortar with 

 about 200 c.c. water. Filter. Dilute the watery 

 extract to 500 c.c. 



Hydrolysis of Ethyl Butyrate by Lipase. 

 Place in each of two test-tubes, A and B, 4 c.c. 

 water, 0.1 c.c. toluene, 1 and 0.26 c.c. ethyl buty-. 

 rate. 2 Cork the tubes tightly. Place them in the . 

 water bath for five minutes, .to bring them to ' 

 the temperature of the bath, 40 C. Add 1 c.c. of r 



1 Toluene is an antiseptic, which prevents the splitting of 

 the neutral fat by bacteria. 



2 Ethyl butyrate hydrolyzes more rapidly than butter fat. 

 It has the further advantage that the amount split by the 

 temperatures employed during the time of the experiment is too 

 small to be measurable. 



