FERMENTATION 213 



enzyme solution, usually 0.1 to 0.2 c.c. $ KOH, 

 should be deducted from the cubic centimetres 

 KOH required to neutralize the fatty acid 

 formed. 1 



Fatty acid will appear in tube A, but not in 

 tube B, in which the enzyme was destroyed by 

 boiling. 



Synthesis of Neutral Fat by Lipase. 1 . Place 

 5 c.c. T ^ butyric acid, 2 c.c. 13 per cent alcohol, 

 1 c.c. diluted glycerine extract of pig's pancreas 

 (or aqueous extract of liver) in each of two test- 

 tubes, A and B. Boil the contents of test-tube 

 B. Seal both tubes. Keep them thirty-six 

 hours at 48.5 C. 



On opening the tubes, A will give a distinct 

 odor of ethyl butyrate; none will be found in B, 

 in which the ferment was destroyed by boiling. 2 



2. Place 5 gms. glycerine, 2 gms. isobutyric 

 acid, 125 gms. water, 1 c.c. neutralized blood serum 

 (or aqueous extract of pig's liver) in each of two 



flask. (Compare Miiller and Kiliani : Kurzes Lehrbuch der 

 analytischen Chemie, 1900, p. 31 and p. 83). 



At 30 (summer temperature) 0.26 c.c. ethyl butyrate weighs 

 0.2300 gram. This quantity, if completely hydrolyzed, would 

 require 39. 7 c.c. ^ KOH. 



1 Kastle and Loeveuhart : American chemical journal, 1900, 

 xxiv, pp. 491-525. Also Loevenhart : American journal of 

 physiology, 1902, vi, pp. 331-350. 



a Kastle and Loevenhart : loc. cit, p. 518. 



