FERMENTATION 231 



This solution, left in the atmosphere, oxidizes 

 slowly to indophenol (violet color). 



Place a drop of a known oxidizer, e.g. ferri- 

 cyanide of potash or potassium bichromate, on 

 the saturated paper. 



The color will change at once, in consequence 

 of immediate oxidation. 



(1) C 6 H 4 (NH 2 ) 2 + C 10 H 7 OH + = 



PARA-PHBNYLENEDIAMINK A-NAPHTHOL 



(2) C.H.NH, C 6 H 4 NH, 



PARA-PHBNYLENEDIAMINK A-NAPHTHOL CgH 4 NHo 



INDOPHENOL 



Each of the combining molecules has been acted 

 upon by a different oxygen atom; hence the oxy- 

 gen molecule must have been split. 



2. Eub the test paper with finely divided tissue 

 from the liver or any other organ. 



Oxidation will occur. 



A drop of blood placed on the test paper is 

 soon surrounded by a characteristically colored 

 ring. 



Extraction of Nucleo-Proteid from Liver. 1 

 Perfuse a fresh liver (dog) with tap water until 

 the washings are no longer colored by haemo- 



1 Spitzer : Archiv fur die gesamrate Physiologie, 1897, Ixvii, 

 p. 616. 



