232 THE INCOME OF ENERGY 



globin. Grind the liver to a pulp and press 

 through several thicknesses of gauze. Add five 

 volumes of distilled water. Allow the mixture 

 to stand twenty-four hours at low temperatures. 

 Remove the opalescent watery extract with a 

 pipette and filter through linen. Demonstrate 

 with the microscope that liver cells are absent 

 from the liquid. Add T 7 ^ HC1 drop by drop until 

 there is no further precipitation, and the super- 

 natant fluid is clear. Since the precipitate redis- 

 solves in acid, use lacmoid as an indicator. Cease 

 when the lacmoid shows a trace of excess. De- 

 cant the precipitate, filter, wash the residue with 

 water. 



Oxidation by Nucleo-Proteid. Place in a wide- 

 necked flask 50 c.c. water containing 0.2 gram 

 of the fresh, brown substance and 10 c.c. hydro- 

 gen peroxide in a small glass cup. The hydrogen 

 peroxide must be neutralized with from 1 to 1.5 

 c.c. YQ NaOH. Connect the flask with the lower 

 end of a eudiometer by means of a bent tube. 

 Shake the flask so that the hydrogen peroxide 

 shall come in contact with the tissue. Oxygen 

 is at once set free. Eead in the eudiometer 

 the oxygen developed from minute to minute. 

 Spitzer found: 



After minutes . . 1234589 16 

 C.c. 2 developed 19 28 41 55 69 85 87 95 



