FERMENTATION 233 



Oxidation about the Nucleus. 1 Introduce the 

 oxidizable solution of a-naphthol and para-phe- 

 nylenediamine (page 230), beneath the cover 

 glass of a fresh preparation of teased thymus 

 or spleen. 



" Granules of the intense greenish-blue oxi- 

 dation product shortly make their appearance 

 within the leucocytes. Their first appearance is 

 typically at the boundary between nucleus and 

 cytoplasm; eventually the latter may become 

 so densely laden as completely to obscure the 

 nucleus. . . . The nucleus is the chief agency in 

 the intracellular activation of oxygen. The ac- 

 tive or atomic oxygen is in general most abun- 

 dantly freed at the surface of contact between 

 nucleus and cytoplasm." 



Glycolysis in Blood. Bernard's Experiment? 

 125 c.c. dog's blood were divided into five equal 

 parts. The sugar in each was estimated as 

 follows : 



Sugar 

 Grams per 1000. 



1. Analysis made at once 1.07 



2. " " after 10 minutes . . 1.01 



3. " " "30 "... 0.88 



4. " " " 5 hours . . . 0.44 



5. " " " 24 " ... 0.00 



1 Lillie : American journal of physiology, 1902, vii, p. 420. 



2 Bernard : Comptes rendus de 1'academie des sciences, Paris, 

 1876, Ixxxii, p. 1406. 



