FERMENTATION 235 



B. Calf's blood 100 c.c. 



Water containing 1.14 gram grape sugar 10 c.c. 



Chloroform 1 c.c. 



Seegen recovered 0.960 gram. 



The chloroform destroys the cells, but fails to 

 check the oxidation. 



Relation of Glycolysis to the Pancreas and the 

 Lymph. 1 Remove the pancreas aseptically from an 

 anaesthetized dog which has fasted thirty-six hours. 

 Estimate the sugar in the urine at intervals of a few 

 hours. 



Sugar will be present in large and increasing quanti- 

 ties, 2 rising even to twenty per cent. 



Inject into the jugular vein 15-20 c.c. of lymph 

 from the thoracic duct of a dog fed a few hours before 

 upon one litre of milk. 



the mixture boil for a few minutes. Filter through a small 

 linen cloth. 



Separation of Proteids. Boil the filtrate. Most of the pro- 

 teids will separate by coagulation. The remainder, it' necessary, 

 may be removed by adding to each 300 c.c. of filtrate, 5 c.c. 

 saturated solution of sodium acetate, and a small quantity of a 

 dilute solution of ferric chloride, neutralizing almost completely 

 with dilute soda solution, and boiling. The ferric chloride will 

 precipitate as ferrous chloride and will carry down the last 

 traces of proteid substances. Filter. Wash with boiling water. 

 (Seegen : Centralblatt fur Physiologie, 1891, v, p. 824.) 



1 Lepine : Comptes rendus de 1'academie des sciences, Paris, 

 1890, ex, p. 742. 



2 Von Mering and Minkowski : Archiv fiir experimentelle 

 Pathologic und Pharmakologie, 1890, xxvi, p. 371. 



