236 THE INCOME OF ENERGY 



The glycosuria will greatly diminish. 



After a few hours, the glycosuria will become once 

 more intense, continuing until death. The quantity of 

 sugar in the blood is also greatly increased. 



Glycolytic Ferment of Pancreas. 1 Remove the 

 pancreas aseptically from a dog immediately after 

 death. Crush it at once in 100 c.c. sterile water 

 containing 0.2 gram sulphuric acid. Allow it to 

 macerate two hours at 38 C. Neutralize the 

 acid with soda, add 0.5 gram pure glucose, and 

 keep the mixture one hour at 38 C. Estimate 

 the sugar. 



The loss will be from ten to fifty per cent. 



When pancreatic extract made without acid is used, 

 the loss of sugar is much less. Probably, therefore, 

 the glycolytic ferment is produced from a zymogen by 

 hyd ration. 



Malt diastase, or salivary diastase, kept three hours 

 at 38 in water containing one tenth per cent sulphuric 

 acid loses the power to change starch to sugar, but ac- 

 quires a glycolytic power. 



If the pancreatic juice which flows upon stimulation 

 of the peripheral end of the vagus (Pavvlow) is treated 

 with dilute acid, 1 : 1000, the amylolytic power is lost, 

 but glycolytic power is acquired. During the excita- 

 tion of the nerve while the juice is flowing the 



1 Lepine : Coraptes reiidus de I'academie des sciences, Paris, 

 1895, cxx, p. 139. 



