FERMENTATION 249 



of 0.1 per cent sodium carbonate solution ; and 

 the myosin in one litre of 5 per cent sodium 

 chloride solution. 



After three hours determine the amount of 



subtract the weight of the filter. There remains the weight of 

 the albumin in 25 c.c. of the original solution. 1 



Preparation of Myosin. Use muscle containing little blood 

 (calf, rabbit, fowl, frog). Hash the muscle. Wash with water 

 until the washings are free from proteid. Remove excess of 

 wash water by pressure. Mix with enough 15 per cent solu- 

 tion of ammonium chloride to cover the mass. After four hours, 

 filter through cloth and then through paper. The filtrate should 

 be clear, opalescent, somewhat thick. Dialyse in running water. 

 As the neutral salt is removed, the myosin will separate in fine 

 flocks (Danilewsky). Redissolve in 5 per cent sodium chloride 

 solution. 



Quantitative Estimation of Myosin. Dialyse 25 c.c. of the 

 saline solution of myosin until the dialysate is free from salt. 

 Wash the precipitated myosin into a tall narrow beaker. When 

 the myosin has settled, decant as much of the supernatant liquid 

 as possible. To the remainder add alcohol in such proportion 

 that the mixture shall contain 80 per cent. After coagulation, 

 filter through a weighed filter. Wash with alcohol and ether. 

 Dry at 100. W T eigh. Subtract the weight of the filter. 



Preparation of Alkali-albumin. Warm the whites of twelve 

 or more eggs with 1 per cent sodium hydrate at 40 C. Filter. 

 Neutralize very cautiously with hydrochloric acid, at first 1 per 

 cent, later 0.1 per cent. The alkali-albumin will be precipi- 

 tated. Allow to stand several hours. Filter. Boil the filtrate. 

 Filter from the fresh precipitate and add residue to first pre- 



1 Literature. KU'HNE: Untersuchungen iiber das Protoplasma, 1864, 

 p. 2. DANILEWSKY : Zeitschrift fur physiologische Chemie, 1881, v. p. 158. 

 HALLIBURTON : Journal of Physiology, 1887, viii, p. 132. KU'HNE and 

 CHITTENDEN : Zeitschrift fur Biologie, 1889, xxv, p. 358. 



