FERMENTATION 251 



needle attached to a burette inject into the 

 loop sufficient egg-albumin solution to distend it 

 slightly. Measure the volume of the solution 

 injected. The content of this solution was found 

 in the course of the experiment on diffusion 

 through dead membranes (page 245). 



Eeplace the loop in the abdomen. At some 

 distance from this loop prepare a control loop 

 with double ligatures in the same way, but leave 

 the control loop empty. Sew up the abdominal 

 wound. 



After three hours, kill the animal (best by 

 puncture of the spinal bulb). Eemove the loops 

 by cutting between the double ligatures. Eapidly 

 wash the outer surface with water, dry the sur- 

 face with filter paper, open the loops, measure 

 the volume of the contents, wash the inner sur- 

 face, add the washings to the contents, and 

 estimate the proteid in a measured portion. 



Perform a similar experiment with solutions of 

 (2) myosin, (3) alkali-albumin, and (4) peptone. 



Compare the results of absorption of proteids 

 through the living intestinal wall with absorp- 

 tion through dead membranes. It will appear 

 that the living cells of the intestinal wall modify 

 absorption so that it does not follow the law of 

 diffusion through dead membrane. 



It is also evident that egg-albumin, myosin, 



