IO BACTERIOLOGY. 



5. Alum- carmine (Grenadier). 



Carmine . . , . % i 



Five per cent, solution of -alum . 100 



Boil twenty minutes, filter when cold. 



6. Ammonia, strong. 



7. Aniline. 



8. Aniline water. 



Distilled water . . .. .100 



Aniline . . . . . . 5 



Shake well and filter emulsion. 



9. Bismarck brown. 



(a) Concentrated solution in equal parts of gly- 

 cerine and water. 



(ff) Aqueous solution. 



Bismarck brown . . . . 2 

 Alcohol . ... . . . 15 



Distilled water .... . 85 



10. Borax carmine (Grenacher). 



Distilled water. . . . .100 

 Borax ...... 4 



Add 4 per cent, carmine; heat twice to all but 

 boiling point, add an equal quantity of alcohol at 

 70, set aside five hours and then filter. 



